All Tools

Comprehensive suite of free online tools for oligonucleotide design, analysis, and quality control. All calculations run in your browser - your data never leaves your device. New to oligo design? Check out our use case examples or read the User Guide for detailed instructions.

Basic Calculators

Essential calculations for oligonucleotide design and handling

Oligonucleotide Properties Calculator

Comprehensive all-in-one calculator: molecular weight, GC content, Tm, OD260, reverse complement, and thermodynamic constants.

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Tm Calculator

Calculate melting temperature using nearest-neighbor thermodynamics. Supports salt and DMSO corrections.

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GC Content Analyzer

Analyze GC percentage with batch processing. Identify extreme compositions and risk levels.

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Molecular Weight Calculator

Calculate molecular weight, extinction coefficient, and concentration conversions. Supports modifications.

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Dilution Calculator

Calculate resuspension volumes, dilutions, and pool-specific concentrations with printable protocols.

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Sequence Analysis

Advanced tools for sequence validation and structure prediction

Secondary Structure Predictor

Predict hairpins, self-dimers, and hetero-dimers. Calculate ΔG for structure stability assessment.

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Batch Sequence QC

Analyze up to 10,000 sequences for quality issues. Detect homopolymers, low complexity regions, and more.

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Quality Control

Tools for assessing oligo pool quality and synthesis parameters

Synthesis Error Rate Calculator

Predict full-length product percentage based on coupling efficiency. Compare with industry standards.

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Pool Uniformity Estimator

Estimate dropout rate and concentration variation. Get QC sequencing depth recommendations.

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Coverage Calculator

Calculate required oligos for CRISPR libraries, tiling applications, and custom coverage strategies.

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Format Conversion

Convert sequences between formats and prepare vendor orders

Sequence Format Converter

Convert between FASTA, CSV, Excel formats. Batch processing with validation and cleanup options.

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Why Use OligoPool.com Tools?

100% Free

No fees, no paywalls, no hidden costs. All tools completely free forever.

Privacy First

All calculations run locally in your browser. Your data stays private.

Scientifically Accurate

Based on published methods (SantaLucia 1998, etc.) with proper citations. View all scientific references and learn about calculation methods in our User Guide.

Lightning Fast

Instant calculations, batch processing up to 10,000 sequences in seconds.

Need High-Quality Oligo Pools?

For professional oligo pool synthesis services, check out our partner Dynegene. High uniformity, competitive pricing, and fast turnaround. Before ordering, use our Batch Sequence QC tool to validate your designs, or follow our oligo pool QC workflow for best practices.

Learn More About Dynegene →